Building antibody diversity

Reshaping Antibody Diversity

Some species mount a robust antibody response despite having limited genome-encoded combinatorial diversity potential. Cows are unusual in having exceptionally long CDR H3 loops and few V regions, but the mechanism for creating diversity is not understood. Deep sequencing reveals that ultralong CDR H3s contain a remarkable complexity of cysteines, suggesting that disulfide-bonded minidomains ma...

Antibody wars: extreme diversity.

Antibody binding loop insertions as diversity elements

In the use of non-antibody proteins as affinity reagents, diversity has generally been derived from oligonucleotide-encoded random amino acids. Although specific binders of high-affinity have been selected from such libraries, random oligonucleotides often encode stop codons and amino acid combinations that affect protein folding. Recently it has been shown that specific antibody binding loops ...

Cellular Basis of Antibody Diversity: Clonal Selection

The specificity of humoral immune responses relies on the huge DIVERSITY of antigen combining sites present in antibodies, diversity which is generated in an antigenindependent fashion. The process by which antigen (during an immune response) stimulates the clonal expansion and differentiation of antibody-forming cells of predetermined specificity is known as CLONAL SELECTION. The basic princip...

A Novel Cytidine Deaminase Affects Antibody Diversity

University of Chicago switch-specific endonuclease then cleaves the doubleChicago, Illinois 60637 strand DNA at random sites within or near Sm and the downstream S region involved in the particular switch. As the switch region repeats are varied, the endonucleAntibody molecules are encoded by immunoglobulin (Ig) ase(s) would not recognize a particular sequence but genes and are produced exclusi...